inverted microscope leica dmi1  (Danaher Inc)

Journal: Biotechnology Reports

Article Title: Indole-alkaloid–rich fraction of Ervatamia coronaria leaf extract regresses breast cancer by inducing apoptotic cell death

doi: 10.1016/j.btre.2025.e00937

Figure Lengend Snippet: DFE induces apoptotic death in MCF7 and MDA-MB-231 cells, as observed by annexin V-propidium iodide (PI) dual staining. [ A.-D. ] Confocal images of MCF7 ( A ) and MDA-MB-231 ( C ) cells pre-treated with indicated doses of DFE (in µg/ml), DMSO, or doxorubicin (1.25 µM, as positive control) for 24 h were stained with annexin V and propidium iodide (PI) (laser scanning confocal microscope images through 63x oil lens). Hoechst 33342 stained the nuclei—scale bar- 25 µm. Bar graphs B and D represent the estimation of panels A and C, respectively. BF, bright fields. The dose-dependent increase of cell death was represented by a scatter plot based on LAS X analysis of the obtained images. Bar graphs representing the relative intensity of annexin V and propidium iodide (PI) in treatment groups. Error bar, mean ± SD ( n = 3); *P<0.05, **P <0.01, *** <0.001, ****P<0.0001.

Article Snippet: Next, the images were taken with a Leica inverted microscope.

Techniques: Staining, Positive Control, Microscopy